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文献和实验Detection of Tetracycline Resistance Genes by PCR Methods
. However, even with all these requisites, for example, no bacteria can be grown from more than 80% of all clinical samples sent to clinical microbiology laboratories (1 ). Besides the cultivation limitations, the cultivation-based determination of an antibiotic resistance profile lacks
Agrobacterium growth and transformation (农杆菌培养及转化)
plasmid that possesses the vir genes needed for T-DNA transfer, but has no functional T-DNA region of its own. Grow at 28-30°C. Store as glycerol stock (800 ml of fresh overnight culture + 200 ul sterile 80% glycerol) at ¬80°C. Transformation
Agrobacterium growth and transformation
needed for T-DNA transfer, but has no functional T-DNA region of its own. Grow at 28-30°C. Store as glycerol stock (800 ml of fresh overnight culture + 200 ul sterile 80% glycerol) at ¬80°C. Transformation of A. tumefaciens with plasmid DNA (binary
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