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文献和实验RNA Purification Protocol for 100-200 mg of Animal Tissue
. Storage at -70°C. Fresh tissue can also be used. Work quickly and keep the sample on ice at all times. 2. Add 100-200 mg frozen ground tissue or fresh tissue to a 15 mL centrifuge tube containing 6 ml RCL Buffer. Homogenize the sample using 5-10
0. (Optional) Before using Oligotex-dT30, I recommend to wash Oligotex-dT30 to remove fine particle of latex. To wash the latex, transfer appropriate amount of Oligotex-dT30 (300 ul of the suspension of Oligotex per 1mg
Concentrating poly(A)+ mRNA Source: Protocol Online Abstract: Simple precipitation method of concentrating poly(A)+ mRNA
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