- ￥3000
- covaris
- 520041
- 2025年07月15日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存：
1
- 供应商：
上海玉博生物科技有限公司
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Single tube confirmation PCR protocol
50 µl total volume *10 x Taq buffer contains: 100 mM Tris-HCl (pH 8.4), 500 mM KCl, 15 mM MgCl2. *The final concentrations are shown in parentheses. *The Taq Polymerase should be added last and PCR mixture should be kept on ice
Single tube confirmation PCR protocol
downstream primer:B,kanB,or D(1 µM) 5 µl Zymolyase treated cells 50 µl total volume *10 x Taq buffer contains: 100 mM Tris-HCl (pH 8.4), 500 mM KCl, 15 mM MgCl2. *The final concentrations are shown in parentheses. *The Taq Polymerase should be added
cells, count and dilute to 4 x 105 cells/ml in cell culture media. 4. Dilute the molecules to be tested to required concentration in cell culture media (Make 2x of the final required concentration). Filter this and serially dilute 1:10, 1:100, 1:1000
技术资料暂无技术资料 索取技术资料
