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文献和实验High Resolution Agarose Gel Electrophoresis
the desired solution volume, add a magnetic stir bar and the calculated volume of buffer at room temperature. Put the flask on a magnetic stirrer and slowly sprinkle the calculated amount of agarose powder into the flask while stirring constantly
substances such as sucrose, glycerol etc. may be added or the experiment can be performed in a sieving medium such as a high concentration of polyacrylamide (PAA) gel. Increased viscosity will also affect the mobility (µ ) the mobility of the proteins
etc. may be added or the experiment can be performed in a sieving medium such as a high concentration of polyacrylamide (PAA) gel. Increased viscosity will also affect the mobility (µ ) the mobility of the proteins. This will make the isoelectric separation longer
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