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文献和实验Dissolve Proteinase K (Sigma Catalog No. P-4914) in dH2O for a concentration of 20mg/ml. 1% BSA/PBS 1g Fraction V BSA in 100ml 1x PBS. Mix in a beaker with low heat. Aliquot in 15ml tubes and store at -20°C. Hematoxylin 75ml Gill's
No. H-1009 (5 ml) Proteinase K Dissolve Proteinase K (Sigma Catalog No. P-4914) in dH2O for a concentration of 20mg/ml. 1% BSA/PBS 1g Fraction V BSA in 100ml 1x PBS. Mix in a beaker with low heat. Aliquot in 15ml tubes
IMMUNOCYTOCHEMICAL IDENTIFICATION OF PROLIFERATING CELLS IN VASCULAR TISSUE (ANTI-BrdU); Diaminobenzadine (DAB) brown detection Sections of paraformaldehyde fixed, OCT-embedded vascular tissue are sectioned at 7 to 10 mm and stored
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