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文献和实验我是用来做筛选IgG型单克隆抗体,用它做二抗,筛到的阳性克隆化和扩大培养后用SIGMA亚类试剂盒鉴定却是IgM,郁闷!!!这个试剂盒能与IGM交叉?是不是抗轻链?我这种方式不对吗?谁知道有较好的抗鼠IgG而与IgM不交叉的抗体?? 试剂盒说明书:Anti-mouse IgG-HRP Antibody FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. R&D Systems, Inc. 1-800-343-7475 2/21/06
Early development of primary motor neurons and somites in Zebrafish Embryos
boundaries. The ZNP-1 and F6 treated embryos will be treated with a secondary antibody, Fluorescent Goat anti-mouse IgG, so that the stained neurons and somites can be observed under a fluorescent microscope. Procedure: 1. Dechorionate 24
ELISA Procedure for Measuring Serum Antibody Titer
. Reagents for Horseradish Peroxidase Detection1. Goat anti-species IgG whole molecule/horseradish peroxidase conjugate that is appropriate for the source of your antiserum. For example, if the antibodies were raised in a rabbit, use goat anti-rabbit IgG
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