scientific polymer特约代理商|707 Po

【转贴】immunofluorescence staining

stock purchased).Wash X 2 @ 5 min. with PEM.Alternately, 0.1 M ammonium chloride diluted in PEM buffer can be used to quench.Incubate for 10 min. Wash X 2 with PEM following this treatment, then 1 X with TBS-T.Counterstain DNA with 1X DAPI diluted in TBS

Staining protocols for placental explant cultures

resin (Agar Scientific Ltd, Taab Laboratories Equipment Ltd) 0.1M sodium cacodylate buffer pH 7.3 +/- 3 mM calcium chloride Method Rinse the explant culture in PBS, then fix in freshly prepared 2.5% glutaraldehyde in 0.1 M sodium cacodylate buffer pH

SSR GEL and Silver Staining Protocol

.i. water. 4. We have recently started using Burst-Pack from Owl Scientific, which is an acrylamide premix including the buffer and catalysts, for our fluorescent gel work and are very pleased with the quality and reproducibility. This would be an option