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RNA Purification Protocol for 10-20mg of Animal Tissue

. Fresh tissue can also be used. Work quickly and keep the sample on ice at all time. 2. Add 10-20 mg frozen ground tissue or fresh tissue to a 1.5 mL microtube containing 600ul RCL Buffer. Homogenize throughly using a microfuge tube pestle (Product

Purification of a Membrane Protein (Ca2+/Mg2+-ATPase) and Its Reconstitution into Lipid Vesicles

., by sonication of membrane proteins with lipids), detergent-based methods are more widely used and appear to be more reproducible (1 ). If a particular protein is to be purified in an active form, then particular attention must be paid to the choice of detergent

Ampicillin(50mg/ml)

  Ampicillin (50mg/ml) Ampicillin Dissolve Ampicillin (Na salt from Sigma, A-9518) in H2 0. Store 1 ml aliquots at 20o C in common freezer for stock solutions   上一篇：PREPARATION