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上海玉博生物科技有限公司
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文献和实验Growth, Maintenance and Transfection of Suspension Adapted 293-EBNA cells
of plasmids containing the viral EBV origin of replication. This protocol describes the instructions for the growth, maintenance and transfection of suspension adapted 293-EBNA cell line using the PEI expression system. Cells can be purchased from ATCC
transfection procedures/reagents, DEAE dextran, calcium phosphate and Effectene™. Aims You will work individually, to perform your transfections Ð two people will work at the same time in the tissue culture facilities in the Wolfson building.
BLOCK-iT Fluorescent Oligo as RNAi Transfection Control
-iT™ Fluorescent Oligo for Cationic Lipid-Mediated Transfection You may use the BLOCK-iT™ Fluorescent Oligo (20 µM or 1 mM stock) with any cationic lipid-based transfection reagent suitable for delivery of Stealth™ RNAi, siRNA, or Dicer-generated siRNA
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