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文献和实验Proliferation Assay: [3H] Thymidine incorporation
cells for 2-4 hours then treat. 5) Six-twenty four hours before cells are to be harvested, add 1 µCi/ml [3H]-TdR to each well. Harvesting: 6) Wash cells 2 times with ice cold PBS. 7) Wash cells 2 times with 5% TCA.
3H-Thymidine Uptake by Cultured Cells
Grow either L cells (Mouse fibroblasts) or chick embryo fibroblasts on coverglasses and then give them H-thymidine for a short period of time. At the end of the labeling period, wash the coverslips in PBSA
of a given molecule on the proliferation of a cancer cell. The methyl-[3H]-thymidine incorporation assay is a widely used, gold standard, method for measuring inhibition of cell proliferation and has been used successfully to screen and optimize potential new
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