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文献和实验E.Z.N.A. Endo-Free Plasmid Mini Kit II Spin Protocol
on desired concentration of final product) Endotoxin-Free Elution Buffer directly onto the column matrix and let it sit at room temperature for 2 minutes. Centrifuge at 13,000 x g for 1 min to elute DNA. This represents approximately 65-85% of bound DNA
E.Z.N.A.® Endo-free Plasmid Mini Kit I Spin Protocol
实验步骤 1. Inoculate 5ml LB/ampicillin (50 ug/ml) medium placed in a 10-20 ml culture tube with E.coli carrying desired plasmid and grow at 37°C with agitation for 12-16 h. It is strongly recommended that an endA negative
E.Z.N.A. Endo-Free BACs, PACs, P1s Protocol
with vigorous shaking (~ 300 rpm). Use a flask with a volume at least 4 times the volume of the culture. 2. Pellet 1.5-5 ml bacteria by centrifugation at 13,000 x g for 3 min at room temperature. Decant or aspirate medium and discard. 3. Resuspend
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