lonza特约代理商|50010 SeaKem® ME Ag

Denaturing Agarose Gel Electrophoresis of RNA

under analysis. RNA molecular weight markers, an RNA sample known to be intact, or both, can be used for this purpose. Ambion''s NorthernMax® reagents for Northern Blotting include everything needed for denaturing agarose gel electrophoresis. These products

E.Z.N.A.® Yeast Plasmid Mini Spin Protocol

III and gently mix by inverting several times until a flocculent white precipitate forms. Centrifuge at $10,000 × g for 10 minutes at room temperature. 9. CAREFULLY aspirate and add the clear supernatant to a clean HiBind® DNA mini column

E.Z.N.A.® Endo-free Plasmid Mini Kit I Spin Protocol

strain of E.coli be used for routine plasmid isolation. Examples of such strains include DH5"® and JM109®. 2. Pellet 1.5-5 ml bacteria in appropriate vessels by centrifugation at 10,000 × g for 1 min at room temperature. 3. Decant or aspirate