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文献和实验Extraction of DNA using DNAzol Reagent
) in DNAzol® Reagent and the genomic DNA is precipitated from the lysate with ethanol. Following an ethanol wash, DNA is solubilized in water or 8 mM NaOH. The procedure can be completed in 10- 30 min with DNA recovery of 70-100%. The isolated DNA can be used
Extraction of DNA using DNAzol® Reagent
实验试剂 1. 100% ethanol 2. 75% ethanol 3. 8 N NaOH 实验步骤 Instructions for Use 1. Lysis/Homogenization 1 ml DNAzol® Reagent 25-50
Quantitation of Protein:Folin - phenol Reagent Method (Lowry Method)
- phenol reagent B : Add l00g of Na 2 WO 4 • 2H 2 O, 25g of Na 2 MoO 4 • 2H 2 O, 700ml of distilled water, 50ml of 85% H 3 PO 4 and 100ml of thick HC1 into a 1.5L round- bottom stoppered flask, mix
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