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Fluorochrome Absorption and Emission Spectra

. BD Cy-Chrome ™ is a tandem conjugate system, with an absorption maximum of approximately 650 nm (Fig. 2), which combines R-phycoerythrin and a cyanine dye (MW 1.5 kDa). When excited by 488-nm light, the excited fluorochrome (R-PE) is able

Fluorochrome Absorption and Emission

. BD Cy-Chrome ™ is a tandem conjugate system, with an absorption maximum of approximately 650 nm (Fig. 2), which combines R-phycoerythrin and a cyanine dye (MW 1.5 kDa). When excited by 488-nm light, the excited fluorochrome (R-PE) is able

Western Blotting Protocols

for primary antibody), labeled with alkaline phosphatase or horseradish peroxidase. Substrate appropriate to the enzyme conjugate (HRP or AP). Appropriate wash buffer: Phosphate-buffered saline (PBST): 10 mM sodium phosphate, pH 7.2, 0.9% (w/v