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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
separately. Isopropyl-β-D-thiogalactopyranoside (IPTG; 200 mg/mL) LB agar LB-ampicillin agar plates Alternatively, LB-kanamycin agar plates can be used (see Step 48). LB (Luria-Bertani) liquid medium Add the appropriate
BAC DNA Isolation from 200 ml Cultures by a Cleared Lysate Method
antibiotic. After incubating at 37 degrees C for 8-10 hours with 250 rpm shaking, the culture is transferred to a 500 ml flask containing 200 ml of the same medium and incubated for 8-10 hours under the same conditions. After harvesting the cells
Recipe for yeast tryptone medium
Keywords: ammonium acetate; Zappe Ammonium acetate Contributor: Zappe, H Related: many protocols Storage: 4 C Ammonium acetate Component stock conc final conc. / 200 ml /1l Ammonium acetate - 7.5M 289g water - - to 200ml
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