Megazyme特约代理商|E-FRMXPD Fructan

Increasing Your RNA Recovery During Tissue or Cell Extraction

in a prechilled mortar and occasionally adding liquid N2 into the mortar to prevent thawing. Once the tissue is ground to a fine powder, the denaturing solution is added to the mortar, and the semi-frozen mixture is stirred. This mixture can then be thawed

96-well Plate Dual Luciferase Assay--96孔板荧光素酶检测

Let sit at Rt for >15’ Suck off the medium of the 96 wells and add 100 ul of mixture (step 5e) by multichanel pipette. Let sit in incubator for >1.5 hours. Add 100 ul of D20F and let it grow for two days. Dual luciferase assay: Reagents:

Fast and reliable mini-prep RNA extraction from Neurospora crassa

otherwise. - Pulverize the mycelium in a mortar with liquid nitrogen - Transfer the powder into a mixture of 0.75 ml lysis buffer (0.6 M NaCl, 10 mM EDTA, 100 mM Tris HCl, pH 8.0, 4 SDS) and 0.75 ml phenol (saturated with 0.1 M Tris HCl, pH 8.0) in a 2 ml