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文献和实验Isolation and Culture of Neurons and Astrocytes from the Mouse Brain Cortex
(such as neurons or glia) to disease progression, but also to investigate the role of neuron–glia interactions during development and pathogenesis of disease. Here, we describe a method to isolate and culture neurons and astrocytes from the mouse cerebral cortex
An In Vitro Trauma Model to Study Rodent and Human Astrocyte Reactivity
regimen. Human astrocytes from fetal cerebral cortex are compared to those derived from cortical biopsies of epilepsy patients (ages 1–12 years old), with regard to growth, purity, and differentiation. This opens a unique
Analysis of HSV Oncolytic Virotherapy in Organotypic Cultures
within this three-dimensional milieu remain preserved. Moreover, with respect to the brain, this system offers the advantage of direct access to brain cells, such as microglia and astrocytes, and circumvents the problems associated with the presence of the blood–brain barrier.
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