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Protocol for Annealing Oligonucleotides

EDTA. 1.Resuspending the Oligonucleotides: Resuspend both complementary oligonucleotides at the same molar concentration, using Annealing Buffer (see note below). For convenience, keep Annealing Buffer volume below 500ml for each oligo

Protocol for Annealing Oligonucleotides

mM Tris, pH 7.5 - 8.0,1mM EDTA.1.Resuspending the Oligonucleotides: Resuspend both complementary oligonucleotides at the same molar concentration, using Annealing Buffer (see note below). For convenience, keep Annealing Buffer volume below 500ml

【精华】vol 687 Chapter 4 巢式PCR侧翼序列测定实验

,in which case, proceed to the next step. 5. Use 0.5 ml of product from the preceding step to template anested 50 ml PfuTurbo?hot start PCR in (see Note 13) 1×reaction buffer including 300 mM dNTPs, 3.75 U PfuTurbo® DNA Polymerase, and 250 nM GSP2 and GSP