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文献和实验Purification of Endotoxin Free mAbs
. If you have larger volumes, or your antibody is present at low concentrations/poor yields, we find it useful to concentrate the supernatants by circulating them through the same type of hollow fibre dialysis cartridges we use for cell growth, allowing the slight
实验试剂 1. Complete Culture Media Formulation for ES (Embryonic Stem) For 500 ml of ES complete media: • 100 ml fetal bovine serum (F2442) • 5 ml 100x non-essential amino acids, NEAA (M7145) • 5 ml
Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional Profiling
of undifferentiated hESC (CD9hi, GCTM?2hi) from the early stages in hESC differentiation (CD9neg or low, GCTM?2neg or low). Curr. Protoc. Stem Cell Biol. 14:1B.7.1?1B.7.15. © 2010 by John Wiley & Sons, Inc. Keywords: human embryonic stem cells
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