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The Role of G-Protein-Coupled Receptors in Adult Neurogenesis

Adult neurogenesis is the process of creating new brain cells during adulthood. This involves several stages including proliferation, migration, differentiation, integration, and survival. G-protein-coupled receptors (GPCRs) regulate

DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE

1954, Pearson et al 1963). The most common such substrate is an indole derivative, 5-bromo-4-chloro-3-indolyl-ß-D-galactoside (X-gal, Holt and Sadler, 1958). When ß-gal cleaves the glycosidic linkage in X-gal, a soluble, colorless indoxyl monomer

Agar Plates for Selection of Clones in Bacteria

C (can be stored in dark at 4C but -20 is usually as simple!) X-Gal: 20 mg/ml in Dimethylformamide (DMF) IPTG: 100 mM Ampicillin: 100 ug/ml (micrograms) Filter sterilize X-Gal is 5-bromo-4-chloro-3-indolyl-beta-D-galactoside. It is hydolysed