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文献和实验with bound (coated) antigen in the presence of known varying concentrations (0–40ug/mL) of the same control peptide antigen (standards) or the unknown (samples) all in a final volume of 100 uL/well in dilution buffer (PBS, 1% BSA, and 0.05% Tween 20
Polyclonal and Monoclonal Antibodies
% ( 1000X ) Protocol: 1) ADSORBTION OF ANTIGEN a) Dilute Ag to 10 ug/ml in PBS. b) Add 100 ul of Ag solution to each well. c) Leave O.N covered with saran wrap, at 4 C. 2) BLOCKING a) Wash unbound Ag by inverting
2) Add 100 ul of the cell suspension to each well. For each condition, use triplicate wells. 3) Add soluble antibody in 100 ul to each well. Titrate antibodies for optimal performance in the assay conditions used
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