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文献和实验SANDWICH ELISA FOR DETECTING SECRETE
A．Materials 1. NUNC-Immuno Plate IIF. 2. Horseradish Peroxidase-Streptavidin (Zymed; 43-4323) 3. PBS or Balanced Salt Solution (BSS). Do not use RPMI or any medium that contains biotin. 4. BSA (1% in PBS). 5. ABTS substrate and substrate
SANDWICH ELISA FOR DETECTING SECRETE
SANDWICH ELISA FOR DETECTING SECRETE Materials NUNC-Immuno Plate IIF. Horseradish Peroxidase-Streptavidin (Zymed; 43-4323) PBS or Balanced Salt Solution (BSS). Do not use RPMI or any medium that contains biotin.
ELISA FOR DETECTING SECRETED ICAM-1
Purpose Materials NUNC-Immuno Plate IIF. Horseradish Peroxidase-Streptavidin (Zymed; 43-4323) PBS or Balanced Salt Solution (BSS). Do not use RPMI or any medium that contains biotin. BSA (1% in PBS). ABTS
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