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上海玉博生物科技有限公司
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文献和实验PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
of No. 30 G 1/2 needle is cut off and then rounded using sharpening stone); 1 ml syringe; Dissecting instruments (fine-pointed scissors, fine forceps); No. 5 forceps (Dumont); Mouth pipette (aspirator mouth piece, latex tubing, blue
Mitochondrial DNA Isolation from Somatic Embryogenic Cell Cultures of Larix (Excerpt from Thesis).
at 3000g for 10 minutes to separate cellular debris, nuclei and proplastids from the mitochondria. Mitochondria are pelleted at 10000g for 20 minutes, resuspended in DNase buffer (0.4M mannitol, 20mM HEPES, pH 7.5, 10mM magnesium chloride) and treated
7.5) mitochondria are further purified by centrifugation in a discontinuous Percoll gradient (45%, 21%, 14% Percoll) at 15000g for 15 minutes. The mitochondria that band are pooled and diluted with resuspension buffer (0.4M mannitol, 20mM HEPES, pH 7.5, 10mM EGTA
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