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文献和实验Subculture of Semi-Adherent Cell Lines
) 0.25% trypsin/EDTA in HBSS, without Ca2+ /Mg2+ (Prod. No. T4049 ) Trypsin (Prod. No. T4424 ) Soybean trypsin Inhibitor(Prod. No. T6414 ) Equipment Personal protective equipment (sterile gloves, laboratory coat
Subculture of Adherent Cell Lines
Materials Media� pre-warmed to 37ºC (refer to the ECACC Cell Line Data Sheet for the correct medium) 70% ethanol in water (Prod. No. R8382 ) PBS without Ca2+ /Mg2+ (Prod. No. D8537 ) 0.25% trypsin/EDTA in HBSS, without Ca2+ /Mg
, and it is broken down by EDTA and calcium-free media. The enzymes collagenase, trypsin and hyaluronidase can also dissociate the desmosome. The nexus , or "gap junction", covers most of the epithelial cell surface. In these areas, the unit membranes appear tightly
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