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文献和实验IMMUNOSCREENING OF LAMBDA-GT11 BACTERIOPHAGE LIBRARIES WITH ANTIBODY PROBES
to the solution. RECIPES: L Broth (LB; Luria-Bertani) 10 g tryptone 5 g yeast extract 5 g NaCl 1 liter water Autoclave IPTG (100mM) 23.8 mg per ml autoclaved water. Weigh
IMMUNOSCREENING OF WITH ANTIBODY PROBES
, rearrange the order once or twice during developing to insure even exposure of all membranes to the solution. RECIPES L Broth (LB; Luria-Bertani) 10 g tryptone 5 g yeast extract 5 g NaCl 1 liter water Autoclave IPTG (100mM) 23.8 mg per ml autoclaved
Southern Blotting(Fred Hutchinson Cancer Research Cente
#2: 2% Blocking Reagent in Buffer #1 Heat to 50-70℃; prepare ~60' in advance; sol'n remains turbid Store at 4℃. Add NaAzide for long term storage. Buffer #3: 100mM Tris-Cl, pH 9.5 100mM NaCl 50mM MgCl2 Antibody: 10 %26micro;l anti-digoxigenin-AP Fab
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