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上海玉博生物科技有限公司
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文献和实验Mouse B cell isolation with magnetic beads
实验概要 Isolate B cells from total mouse cells Praparation: 1.Enough MACS; put RPMI into water bath. 2.Put some MACS in 15 ml tube or 1.5 ml tube from cell room
with 5 ml of PBS and add to gradient. Centrifuge at 300 g for 40 minutes, room temperature. Collect the B cells at the interface. Wash 3 times with PBS. Suspend the SRBC-T cell pellet. Centrifuge at 300 d for 10 minutes. Aspirate all of the supe
Reproducible purification of DNA and RNA in 96-well format. Genomic DNA and total RNA were purified from mouse tissues using the AllPrep DNA/RNA 96 Kit. A Purified DNA samples were analyzed on a 0.8% agarose gel. B
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