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文献和实验Using Soil DNA Kit (D5625) to Clean Up DNA containing inhibitors
5. Optional: If RNA-free DNA is required, add 2ìl RNase A (25mg/ml) and mix throughly by vortexing for 10 seconds. Incubate at 37EC for 10 minutes. 6. Add 100μl of equilibration buffer to HiBind DNA Column(inserted into a 2 mL collection tube
实验试剂 1. Sterile deionized water (or TE buffer) 2. Absolute (or 95%) ethanol 实验设备 1. Sterile 1.5 mL centrifuge tubes. 2. Protective eye-ware 实验步骤 1. MicroElute™ DNA
"Glassing Out" DNA Using the Geneclean Kit
up and down. Incubate the tube in a 50 degree water bath for 3 minutes.\ The DNA does not bind to the silica matrix in the absence of salts and is eluted during this step. 13. Spin for 30 seconds in a microcentrifuge to make a solid pellet. Carefully remove 20 ul
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