biocision特约代理商|BCS-116 Item No
. BCS-116, Holds 96 x 1.5 mL or 2.0 mL microfuge tubes, A-H and 1-12 row and column indexing 7.00 lbs- ￥3000
- Biolegend
- 613404
- 2025年07月16日
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文献和实验% ethanol. Dry pellet and resuspend in 150µl water.III.Repairing ends of the sheared DNAAdd the following components to a microfuge tube:Sheared DNA (10-50g)dH2OdNTPs (25mM mixture of 4 dNTPs)BSA (10mg/ml)10X T4 DNA Polymerase BufferT4 DNA PolymeraseTotal
. Lay the cover slip on the top of an upside down lid of a small worm plate with two strips of lab tape across it. This holds the cover slip at about the same height as the worms on a plate so that you don't have to focus around too much when switching
on the microinjection needle. Using the fine controls, carefully lower the injection needle towards the stage until it is in the same focal plane as the micropipette. At this low power, you can't see the actual tip, so you may have to try the 40X objective
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