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文献和实验P. , Lin, H. C. , Wang, S. S. , Callaway, J. , and Wilcox, G. ( 1 987) Characterization of the Erwinia carotovora peLB gene and its product pectate lyase. J . BacterioL 16 9, 4379-4383. 34. Yanisch-Perron, C. , Vieira
centrifuge. Total RNA was precipitated with 2 vol of 2-propanol (Fluka) at −20°C for at least 16 h. The RNA pellet was washed with 75% ethanol (Merck), dried, and dissolved in DEPC-treated water (Ambion). The RNA was quantified with Quant-iT™ RiboGreen® RNA
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
. Isacson. 1992. Authentic and artifactual detection of the E. coli laxZ gene product in the rat brain by histochemical methods. Brain Research 16 :311. Snyder, E.Y., D.L. Deitcher, C. Walsh, S. Arnold-Aldea, E.A. Hartwieg, and C.L. Cepko. 1992
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