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文献和实验Introduction to the EMSA (Gel Shift) Technique
by the relatively low ionic strength of the electrophoresis buffer used. Upon entry into the gel, protein complexes are quickly resolved from free DNA, in effect freezing the equilibrium between bound and free DNA. In the gel, the complex may be stabilized
Microfluidic Primer Extension Assay
, it was found that miRNAs also exist in a broad range of body fluids (Clin Chem 56:1733–1741, 2010). A major challenge still is the efficient and specific detection of miRNAs. The short length of miRNAs, with only 17–27 base pairs, comes with technical difficulties
Labeling Tubulin and Quantifying Labeling Stoichiometry
to tubulin. We have used it successfully to derivatize tubulin with succinimidyl esters of biotin, digoxigenin, and a wide range of fluorochromes such as tetramethylrhodamine, X-rhodamine, fluorescein, Oregon Green, Cy3, Cy5 and C2CF (bis-caged
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