QIAGEN凯杰 代理商|981103 Strip Tube

Urea Lysis Protocol

(depending on # of cells and strip size) lyse at room temperature for 30 – 45 min,vortexing every 10 min transfer lysate to μltracentrifuge tubes and spin at 50000 RPM at 21℃ for 90 min apply the supernant to a Qiagen QIAshredder (cat#79654),spin at 14000

cDNA Library Screening Protocol - ZAPII

C for 2 hr with lid slightly ajar to complete drying. 2) Pick a single XLI-Blue MRF' colony with a sterile toothpick to inoculate 50 ml of LB broth containing 0.5 ml of 20% maltose and 0.5 ml of 1M MgSO4 in a sterile 250 ml flask. Grow overnight

Worm genomic Southern blots

less than ~200 µl worms here, if you have more then split into two tubes. Should get 200 µl worms from 1-3 large plates). Move to a 1.5 ml eppendorf tube. At this point, can throw the worms into the -20° freezer to synchronize preps of different strains. Freezing