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文献和实验v468 Chapter 6 细胞内结肠腺瘤样息肉蛋白核定位以及细胞质检测
of completedexperiment). 8. The membranes can be re-probed with other antibodies as afractionation or loading control. Fig. 6.1. Detection of APC by biochemical assay and its confirmation using RNAi. AEquivalent amounts of cytoplasmic or nuclear extracts
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
phosphorylation of b-catenin, but not CRMP2. A Lysates of SH-SY5Y cellsincubated with conditioned medium from control (–) or Wnt3a-expressing (+) L cells (1 h) were subjected to SDS-PAGEand transferred to nitrocellulose membranes. Membranes were probed
.D.600 equivalents). Add appropriate volume of primary antibody. Incubate overnight 4°C on Nutator. I usually set up duplicate IPs. Note: Always do NO Ab control. Also, if adding competitor antigen, do so several minutes before adding Ab
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