RPI特约代理商|C32200 Cellulase [Ono

Cellulase assays

NaCl solution for at least 10-15 minutes. Wells containing cellulase should have a clear zone around them, and the diameter of the zone gives a measure of the cellulase activity in that well.   Viscosity reduction method  

Cellulase assays

°C. After the incubation period is finished, use tap water to rinse out the contents of the wells, and then flood the dishes with Congo red solution for 15 minutes. Then rinse the dishes with 1 M NaCl solution for at least 10-15

细菌cellulase活性的检测方法

cellulase活性的检测方法很多，包括： 1.棉线切断法； 采用Monod 恒温振荡器,将缝纫机线(15 支纱) 的一端浸入含有5 ml 酶液的试管中, pH 5. 0 ,40 ℃,72 r·min - 1振荡测定纱线切断所需时间,以此来得到酶活力。 2.滤纸崩溃法 采用Monod 恒温振荡器,将两片滤纸(1 ×1 cm) 加入装有5 ml 酶液试管中,40 ℃,72 r·min - 1 ,pH 4. 0 。计算滤纸完全崩溃所需要的时间,求得酶活力。