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文献和实验PCR引物设计和优化(PCR PRIMER DESIGN AND REACTION OPTIMISATION)
a stable double-stranded, anti-parallel "hybrid" molecule. One may make nucleic acid (NA) single-stranded for the purpose of annealing - if it is not single-stranded already, like most RNA viruses - by heating it to a point above the "melting temperature
Analytical Ultracentrifugation: Sedimentation Velocity Analysis
, W.B. 1968. Theory of transport of interacting systems of biological macromolecules. Adv. Enzymol. Relat. Areas Mol. Biol. 30:139‐177. Casassa, E.F. and Eisenberg, H. 1964
DNA extraction from blister rust aeciospores and urediniospores using dry grindi
of Cronartium quercum f.sp. fusiforme. Phytopathology 83:388-392. Hamelin, R. C., J. Beaulieu, and A. Plourde. 1995. Genetic diversity in populations of Cronartium ribicola in plantations and natural stands of Pinus strobus. Theor. Appl. Genet. 91:1214-1221
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