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文献和实验of antibodies, which might negatively effect cell growth.Our first procedure will involve the simple transfer of an established culture from a suspension culture. An aliquot will be removed from a commercially available cell line and transferred to prepared
IMMUNOHISTOCHEMISTRY ON SCHNEIDER LINE 2 CELLS
2-3X in PBS. 3. Rinse 1X in 100mM NaHPO4, pH 7.2-7.4 (diluted from 1M stock). 4. Fix 15-30' in 2.5% paraformaldehyde-NaHPO4. 5. Wash 5X in PBS (10' total). 6. Permeabilize cells in 0.25% saponin-PBS for 10' @ RT. 7. Quench cells
Size‐Exclusion Chromatography with On‐Line Light Scattering
the use of size?exclusion chromatography with on?line light scattering, UV absorbance, and refractive index detectors (SEC?LS/UV/RI) to determine: (a) the molecular weight of simple proteins containing no carbohydrates, (b) the molecular weight
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