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FAM caspase 8 and 9 binding assay for embryos

is available.     MATERIALS Preparation of 150X FAM Reagent Frozen Aliquot Preparation (Store frozen)   Add 50 µl of DMSO to one vial of lyophilized reagent.  Mix until dissolved.  Aliquot 10 µl into tubes for later use.  Freeze

Methylated CpG Island Amplification

hybridization solution (e.g. BLOTTO) Random-primed DNA labeling kit Wash solutions (Wash1 2xSSC, 0.1%SDS; Wash2 0.1XSSC, 0.1%SDS) 1.2. RDA and Cloning PCR Products 3 X EE buffer : 30 mM EPPS (SIGMA) pH 8.0, 3 mM

Methylated CpG Island Amplification (MCA)

Methylated CpG island Amplification   Protocol written by Minoru Toyota 2. Materials 2.1. MCA Restriction enzymes SmaI, XmaI T4 DNA ligase Taq DNA polymerase 10X PCR reaction buffer: 670mM Tris-HCl, pH