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文献和实验The T-Dependent Antibody Response to Keyhole Limpet Hemocyanin in Rodents
production) to an introduced antigen (i.e., keyhole limpet hemocyanin (KLH)) in rats or mice treated with a chemical (e.g., a known immunotoxicant and/or a new drug candidate). The TDAR model of competent immune function requires the participation of multiple
Preparation Of Peptide-KLH Conjugates For Immunization
will not couple well. You may need to regenerate the thiol groups by reducing the peptide with excess DTT and running a P2 column.2. Coupling of Peptide to KLHThis recipe is for two bunnies for about five injections per bunny(1) Weigh out 100mg of keyhole limpet
Preparation Of Peptide-KLH Conjugates For Immunization
for about five injections per bunny Weigh out 100mg of keyhole limpet hemocyanin (KLH). Dissolve in 2ml water. It generally takes about 4 hours to dissolve- you will need to sonicate and vortex. Be patient and put on a rotator at 4 deg C. Dialyze against 2l of 0.1M
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