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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
serum, obtained from the same species as the secondary antibody. Depending on the method employed, use anti-rabbit IgG (Fc) horseradish peroxidase (HRP) conjugate (1:100 dilution), a biotin-conjugated secondary antibody, or a fluorescence-conjugated
IMMUNOHISTOCHEMISTRY ON WHOLE MOUNT EMBR
, although my current view is that this is not necessary. I use Tago G anti- Ms, Rb and Rt. I use HRP-avidin and AP-avidin from BRL @ 1:2000 and 1:1000, respectively. Add avidin-enzyme conjugate in PBT, rotate 30''. Wash 3X in PBT with a final wash in AP
IMMUNOHISTOCHEMISTRY ON WHOLE MOUNT EMBRYOS
conjugate in PBT, rotate 30'. Wash 3X in PBT with a final wash in AP buffer if using AP-conjugate.. The AP staining conditions are as described in the BRL handout that comes with the conjugate. Although the AP stain is more sensitive than HRP
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