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文献和实验SYBR Green Quantitative PCR Protocol
12μl of master mix for each well, plus some excess1 Rxn: 10μl SYBR Green Mix 52 Rxn: 520μl SYBR 0.4μl Forward Primer (10μM stock) 20.8μl F
the SYBR green dye, it may well be inhibiting your reaction. What is concentration of SYBR Green in your final PCR reaction mix? There may also be contaminants in the SYBR Green that may be inhibiting the PCR reaction. You could perform an old
Realtime RT-PCR problem - no product-Real-Time PCR
amplification. You may need to titrate the SYBR green dye, it may well be inhibiting your reaction. What is concentration of SYBR Green in your final PCR reaction mix? There may also be contaminants in the SYBR Green that may be inhibiting the PCR
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