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文献和实验Overview of Formation of G‐Quadruplex Structures
., Bialis, T., Jones, R.A., and Yang, D. 2006. Human telomeric sequence forms a hybrid‐type intramolecular G‐quadruplex structure with mixed parallel/antiparallel strands in potassium solution. Nucleic Acids Res. 34: 2723‐2735
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
solution ( 36.5%; Sigma-Aldrich 33220) Glycine (2 M; Fisher BP381) Glycogen (Roche) H2 O, nuclease-free Immune complex wash buffers (high-salt and low-salt) Prepare the high-salt and low-salt versions of this buffer
-S in 500 ml 1% acetic acid(Fisher# BP103-50)Developing Solution100 mM Tris 9.5 15 ml 1M Tris 9.5100 mM NaCl 3 ml 5M NaCl5 mM MgCl2 0.75 ml 1M MgCl2up to 150ml with QTo use, add 66 ml NBT and 50 ml BCIP to 15 ml of Developing Solution.NBT (sigma N
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