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文献和实验Standard hybridization technique
The standard solution typically used for both pre-hybridisation and hybridisation is based
as otherwise all available Mg2+ is chelated out of solution and enzyme reactivity is adversely affected. Any increase in dNTPs over 200uM means [Mg2+] should be re-optimised.AVOID USING EDTA-CONTAINING BUFFERS AS EDTA CHELATES Mg2+Low primer, target, Taq, and nucleotide
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
The easiest way to heat these solution is to submerge large, double zipper plastic bags in water bath. The temperature used varies for different probes and samples. Make sure you know what tempt to use. 60 C is standard for homologous DNA/DNA/RNA hybs
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