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文献和实验the collection tubes for next step. 6) Add 700 ul of SPW Wash buffer diluted with ethanol to the HiBind™ MicroElute™ DNA column and spin at 10,000 x g for 1 minute. Discard the flow-through and place the column back into same 2mL collection tube.
) and centrifuge at 8000-10,000 x g for 1 min at room temperature. Discard the liquid and re-use the collection tube. For volumes greater than 700 ul load the column and centrifuge successively, 700 ul at a time. Each HiBind® extraction column has a total capacity
but thoroughly, immediately after addition of Buffer N3. The solution should become cloudy. Centrifuge for 10 min at 13,000 rpm (~17,900 x g) in a table-top microcentrifuge. A compact white pellet will form. Apply the supernatants from step
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