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- invitrogen
- K182002
- 2025年07月05日
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文献和实验Crystallization of Kinesin Family Motor Proteins
diffusion2 microliters of protein (15 mg/ml) + 2 microliters of reservoir buffer (RB1) composed of 30% w/v PEG4000, 100 mM Tris-HCl pH 8.5, 200 mM sodium acetate, 8% w/v sucrose, 4 mM ADP, 10 mM MgCl2, equilibrated against RB1 for 5 dKikkawa et al., 2001KIF
or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture
In Vitro Transcription Assay (Run-off Assay) using Permeabil
Prepare in ddH2 O 100 mg/ml Phosphocreatine, disodium salt; hydrate Yeast tRNA Solution 50 mM EDTA, pH 8.0 1% (w/v) Yeast tRNA
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