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文献和实验for protein quantitation and structural characterization (see Note 1 ). Many of the existing methods for removing buffers and salts from proteins, such as precipitation methods or reverse-phase high-performance liquid chromatography (RP-HPLC) result
Utilizing Peptide SPOT Arrays to Identify Protein Interactions
of synthesized peptides 12? to 18?amino acids long, with overlapping sequences that cover the entire sequence of a protein, covalently linked to a solid support. This unit describes how to construct peptide SPOT arrays, biotinylate recombinant proteins
Purification of Extracted Peptides for Structural Analysis
The continued modifications in peptide/protein sequencer hardware, derivatization and coupling chemistry, reagent delivery, and component detection provide the protein/peptide chemist with the tools to determine primary
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