- ￥3000
- Merck Millipore
- SD1P014M04
- 2025年07月06日
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文献和实验Fluorescein labeling of proteins
.CFSE has high reactivity with aliphatic amines,low reactivity with aromatic amines,including tyrosine. Reagents CFSE Carboxyfluorescein succinimidyl ester (Molecular Probes C-1311).Store desiccated at -70 ℃ DMSO Dimethylsulfoxide,anhydrous PBS
Fluorescein labeling of proteins
CFSE Carboxyfluorescein succinimidyl ester (Molecular Probes C-1311). Store desiccated at -70 °C DMSO Dimethylsulfoxide, anhydrous PBS Phosphate buffered saline, pH 7.4 * Protein to be labeled, purified, ~1 mg/ml in PBS * Column for gel
Phycoerythrin conjugation protocol
DMSO Dimethyl sulphoxide, anhydrous SPDP 3-(2-pyridyldithio)propionic acid N-hydroxysuccinimide ester (Sigma) SMCC Succinimidyl trans-4-(N-maleimidylmethyl)cyclohexane-1-carboxylate
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