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文献和实验A Cell-Detachment Solution Can Reduce Background Staining in the ELISPOT Assay
a background and then counted as specific spots. We have tested a cell detachment reagent, Accumax™, and found that it may be used to remove a large number of cells adhered to the microplate membranes. Accumax was tested in 16 different ELISPOT assays
Cell Surface Immunofluorescence Staining Protocol
实验试剂 Cell Staining Buffer [ Details:BioLegend Cat. #420201] Red Cell Lysis Buffer [ Details: BioLegend Cat. #420301] 7-AAD Viability Staining Solution[ Details: BioLegend Cat. #420403] TruStain FcX™[ Details: anti
, like CANDOR’s PlateBlock™, are a possible solution to the discussed false results. Problems of blocking reagents Efficient surface blocking is an important and complex topic to be solved. Yet, many assays employ poorly defined blocking reagents
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