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文献和实验Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells
We typically work with tissue culture, primary mammalian cells, and cell extracts, but the protocols can be adapted to other systems, such as whole embryos or lower eukaryotes. The cytoskeleton is very dynamic and sensitive to changes in both the chemical
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells
and sensitive to changes in both the chemical and mechanical environment. Optimal conditions for fluorescence of proteins of the actin and tubulin cytoskeleton are based on: preserving cell structure; properties of individual cytoskeletal proteins
Site-directed hydroxy radical mapping of nucleosome positions in vitro
Introduction The dynamics of chromatin structure is becoming an area of increasing interest. Both thermal energy and ATP-dependent chromatin remodelling enzymes can alter nucleosome structure and positioning. In addition, modifications
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