biorad特约代理商|1511901 Gel Filtra

Determination of Molecular Size by Size‐Exclusion Chromatography (Gel Filtration)

of purity, and study of interactions between proteins. In this unit protocols are provided for size?exclusion high?performance liquid chromatography (SE?HPLC) and for conventional gel filtration, including calibration of columns (in terms of the Stokes

Determining the Subunit Structure of Phosphodiesterases Using Gel Filtration and Sucrose Density Gradient Centrifugation

Size-exclusion chromatography (gel filtration) is a widely used method to determine the molecular weight of a protein. Often, the elution volume of several standard proteins is plotted against their known molecular weight to generate

Size and Shape of Protein Molecules at the Nanometer Level Determined by Sedimentation, Gel Filtration, and Electron Microscopy

of the unknown protein SMC protein from B. subtilis was determined by gel filtration. Fig. 3  Determination of R s of BsSMC by gel filtration. The column was calibrated by running standard proteins BSA, catalase, and thyroglobulin