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文献和实验Viscosity Polymeriztion of Microtubules
Compute the amount of GTP (M.W. 523) needed to make a final concentration 0.5 mM GTP when added to 40 ml. of brain extract in microtubule buffer. Likewise, compute the amount of dry ATP (M.W 507) needed to make a final concentration
CHART 1. take ~350 gram rabbit skeletal muscle 2. extract at +4 oC with 0.1 M KCl and filter 3. (take residue) extract at +4 oC with 50 mM NaHCO3 and filter 4. (take residue) extract at +4 oC with 1 mM EDTA, pH 7.0 and filter
GST-fusion protein injection into rabbits
. Combine eluted fractions (El1+El2) in 50 ml falcon tube, freeze at -80oC, close with puncture plastic cap. Lyophilize over-night. Resuspend in 0.5-1 ml of DDW, spin, and transfer to a small tube. Store at -20oC. Rabbit Screening It is important
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