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上海玉博生物科技有限公司
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文献和实验Mag-Bind Blood DNA Maximum Yield Protocol (2ml-6ml)
实验步骤 1. Place a empty, uncapped 50 ml conical tube into a 50ml tube holder. 2. Add 1-6 ml blood sample to 50ml tube. 3. Add indicated volume of PBS, MSL, Proteinase K and RnaseA showed in the table to the sample. Mix
Eppendorf 管中,吸取500μl AW buffer 放入柱子中心,10000×g 离心1min (洗涤DNA ,除去盐分); (j)重复一次,吸取500μl AW buffer 放入柱子中心,10000×g离心2min (确保除去所有的缓冲液),在使用前2min 从温育中取出 AE buffer ; (k)将柱子放置于一新的2ml Eppendorf 管中,吸取100μl AE buffer 放入柱子中心,10000×g离心1min (洗脱DNA ); (l)重复(K)步骤一次; (m)将装有
DNA Purification from Blood or Body Fluids
genomic DNA is required, 4 µl of an RNase A stock solution (100 mg/ml) should be added to the sample before addition of Buffer AL. Note: It is possible to add QIAGEN Protease (or proteinase K) to samples that have already been dispensed
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